Experiment on Changes in Serum Levels of Irisin

Experiment on Changes in Serum Levels of Irisin Khaled Abdelfattah Abdelhamid Abulfadle, MD   Serum irisin level changes after propylthiouracil treatment of L-thyroxine induced hyperthyroidism in rats ABSTRACT Background: Thyroid hormones play an essential role in lipid, protein and carbohydrate metabolism; and they regulate thermogenesis and basal metabolic rate. Irisin is a myokine that is secreted as a product of fibronectin type III domain containing 5 (FNDC5) was shown to be present in many tissues, including the thyroid tissue. Objective: To investigate the possible changes in serum levels of irisin that occurred in a rat model of hyperthyroidism and the effects of use of propylthiouracil (PTU) in its treatment. Design: 18 male albino rats were divided into 3 groups. Group I was the intact control. Rats of group II and III received daily 0.3 mg/kg subcutaneous injection of L-thyroxine (LT4) for 12 consecutive days. On the thirteenth day, animals of group III (Hyperthyroid PTU treated) received 5 ml/kg i.p. injection of propylthiouracil (PTU) along with an equivalent amount of LT4 as administered to group II animals for more 15 days. Animals of group II (Hyperthyroid control) continued to receive LT4. On the day of termination (28th day of starting LT4 treatment), overnight (12hrs) fasted animals were sacrificed by decapitation under ether anesthesia. Results: In the hyperthyroid control group, there was a significant decrease in serum level of TSH (P0.05) in serum levels of TSH, T4, T3 irisin, and, BMI in hyperthyroid PTU group in comparison to that in the intact control group. In the hyperthyroid control group, there was a significant increase in the serum level of both glucose (P0.05) in the hyperthyroid PTU treated group in comparison to that in the intact control group. In the hyperthyroid control group, there was a significant decrease in serum levels of both TC (P0.05) in serum levels of both TG and LDL-C in comparison to that in the intact control group. Conclusion: This study showed a significant increase in serum levels of irisin in L-thyroxine induced hyperthyroidism in rats. On using PTU, serum levels of irisin, thyroid hormones, metabolic parameters and BMI were significantly changed in comparison to the hyperthyroid control group. Keywords: Irisin, T3, T4, TSH, Propylthiouracil, Hyperthyroidism. Introduction: Irisin is a myokine that is secreted as a product of fibronectin type III domain containing 5 (FNDC5) in the skeletal muscle (Samy, Ismail et al. 2015, Jin Zhang 2017, Nathan C. Winn 2017). FNDC5, which is the precursor of irisin was shown to be present in many tissues, including the thyroid tissue (Huh, Panagiotou et al. 2012). It is secreted by a peroxisome proliferator-activated receptor ÃŽÂ ³ (PPARÃŽÂ ³) coactivator 1ÃŽÂ ± (PGC1-ÃŽÂ ±) after an exercise (Aydin 2014). Irisin was found to have an anti-obesity effect as it replaces the white fatty tissue with brown fatty tissue by the increase of uncoupling protein-1 (UCP1) resulting in oxygen consumption and stimulation of thermogenesis (Bostrà ¶m, Wu et al. 2012, Castillo-Quan 2012, Roca-Rivada, Castelao et al. 2013, Jin Zhang 2017, Nathan C. Winn 2017). Stengel, Hofmann et al. (2013) found that irisin level was identified to be high in obesity and to be low in anorexia nervosa. On the other hand, thyroid hormones pla y an essential role in lipid, protein and carbohydrate metabolism; and they regulate thermogenesis and basal metabolic rate (Hulbert 2000). Also, hyperfunction of thyroid gland is a common endocrine disorder which is accompanied by metabolic imbalance, oxidative stress and abnormal energy homeostasis (Erdamar, Demirci et al. 2008). There were some controversies in the results of studying the effect of thyroid hormone level changes on serum levels of irisin as Yalcin, Akturk et al. (2016) found an increase in serum irisin levels in hyperthyroidism and they suggested that it might contribute to altered energy metabolism. Also, Samy, Ismail et al. (2015) stated that hyperthyroidism was associated with up-regulation of serum irisin in male rats, probably as a response to myopathy and/or oxidative damage observed in both conditions. Moreover, Ruchala, Zybek et al. (2014) reported a borderline elevation of serum irisin in hyperthyroid versus hypothyroid patients. On the contrary, Panagiot ou, Pazaitou-Panayiotou et al. (2016) stated that changes in thyroid hormone levels did not affect circulating irisin levels in humans. Also, Stengel, Hofmann et al. (2013), Ellefsen, Vikmoen et al. (2014) and Gouni-Berthold, Berthold et al. (2013) reported lack of associations between levels of irisin, TSH and/or thyroid hormones. On the other hand, Zybek-Kocik, Sawicka-Gutaj et al. (2016) stated that irisin concentration was changed during prolonged thyroid function impairment. Also, AteÃ…Å ¸, Altay et al. (2016) found that, in hypothyroidism, there was a positive correlation between serum irisin levels and thyroid stimulating hormone (TSH) while a negative correlation was found between irisin levels and thyroxine (T4). Thus, this study was conducted to investigate the possible changes in serum levels of irisin that occurred in a rat model of hyperthyroidism and the effects of use of propylthiouracil (PTU) in its treatment. Materials and Methods: Animals preparations and experimental protocol: In the Department of Physiology, Faculty of Medicine, Zagazig University, this experimental study was done from 6th of November 2016 to 28th of December 2016. 18 adult male albino rats, age and weight matched (170-195 gm), were purchased from the animal house of Zagazig University and were housed at 20-22 °C on a 12-h light-dark cycle and for 5 days, to achieve acclimatization, they were supplied with tap water and a standard chow diet. Rats were divided into 3 groups of 6 each. Group I animals served as the intact control, whereas those of group II and III received daily subcutaneous injection of L-thyroxine (LT4) (Sigma, MO, USA) at a dose of 0.3 mg/kg for 12 consecutive days (Panda and Kar 2007). On the thirteenth day, animals of group III received propylthiouracil (PTU) (Sigma, MO, USA) 10 mg/kg intraperitoneally (Panda and Kar 2007) along with an equivalent amount of LT4 as administered to group II animals. PTU was intraperitoneally (i.p.) injected, in a volume of 5 ml/kg, dis solved in saline (Kim, Kim et al. 2012). Experiment was continued for more 15 consecutive days (from 13th LT4 treatment). Animals of group II continued to receive LT4 and acted as hyperthyroid control. Intact control rats were injected with 5 ml/kg/day subcutaneously with saline. On the day of termination (28th day of starting LT4 treatment), overnight (12hrs) fasted animals were sacrificed by decapitation under ether anesthesia. Blood from each animal was collected and by its centrifugation at 3000 rpm for 15 minutes, serum was separated and stored -20oc till the time of assay of different biochemical parameters (Kumar, Kar et al. 2014). PTU was used to treat hyperthyroidism by decreasing the amount of thyroid hormone produced by the thyroid gland (Nakamura, Noh et al. 2007), and it also inhibited the enzyme 5-deiodinase, which converts thyroxine (T4) to the active form triiodothyronine (T3) (Bahn, Burch et al. 2009). Also, it inhibited LT4-induced hyperthyroidisms in rats (Panda a nd Kar 2007). Body mass index (BMI) changes: BMI in gm/cm2 was calculated in the different groups, just before rats were sacrificed (under ether anesthesia), by dividing Body weight (gm) on Length2 (cm2) (Novelli, Diniz et al. 2007). A graduated (in centimeters) metal ruler was used for measuring the rat length by putting the zero end at the rat anus and recorded the reading which reached by the nose. BMI was used as a measure for the change in body weight of rats (Novelli, Diniz et al. 2007). Figure-1: Experimental design. Rats were divided into 3 groups. Group I was the intact control. Rats of group II and III received daily subcutaneous injection of levothyroxine (LT4) for 12 consecutive days. On the thirteenth day, animals of group III (Hyperthyroid PTU treated) received i.p. injection of propylthiouracil (PTU) along with an equivalent amount of LT4 as administered to group II animals for more 15 days. Animals of group II (Hyperthyroid control) continued to receive LT4. On the day of termination (28th day of starting LT4 treatment), overnight (12hrs) fasted animals were sacrificed by decapitation under ether anesthesia. Measurement of serum parameters: Serum level of irisin was estimated (in ng/ml) using an irisin rat ELISA assay kit following the manufacturers instructions (Catalog# EK-067-52; Phoenix Pharmaceuticals Inc., Burlingame, California, USA) with a detection range of 0.1-1000 ng/ml. Quantitative immunoassaying of T3, T4 and TSH using commercially available kits as recommended by the manufacturer (cat#SE120091, SE120090, SE120135, ELISA Kits, Sigma-Aldrich). Both serum levels of total cholesterol (TC) and triglycerides (TG) were estimated using enzymatic colorimetric methods. Serum high density lipoprotein-cholesterol (HDL-C) was assayed using NS Biotec HDL-precipitating reagent. Serum low density lipoprotein-cholesterol (LDL-C) was calculated using the Friedewald formula: LDL-C (mg/dl) = [(TC) (HDL-C) ( (Friedewald, Levy et al. 1972) Estimation of both serum glucose levels, using an automated analyzer (YSI 2300; YSI Life Sciences, Yellow Springs, OH), and serum insulin levels, using ELISA kits (RD Systems, Minneapolis, MN), were done. For assessing insulin resistance (IR), the homeostasis model assessment-IR (HOMA-IR) index was calculated by: HOMA-IR = (Nayak, Hillemane et al. 2014). There is a direct relation between insulin resistance and the value of HOMA-IR (Bonora, Targher et al. 2000). Statistical analysis: By the use of IBM SPSS Statistics for windows package version (24), data were analyzed and described as Mean ± SEM. For calculation of the statistical significance, One-way ANOVA and Tukey HSD post hoc test for multiple group comparison were used. P†°Ã‚ ¤0.05 indicated significance. Results: Table-1: Changes in serum levels of TSH, T4, T3 irisin, and, BMI among different groups Intact control Hyperthyroid control Hyperthyroid PTU treated TSH (à ¯Ã‚ Ã‚ ­IU/L) 2.82 ±0.12 0.81 ±0.05a 2.68 ±0.14bc T4 (à ¯Ã‚ Ã‚ ­g/dl) 5.33 ±0.14 16.2 ±0.2a 5.36 ±0.18bc T3 (ng/ml) 1.29 ±0.04 3.52 ±0.11a 1.36 ±0.04bc Irisin (ng/ml) 401.67 ±5.65 599.17 ±6.82a 409.5 ±6bc BMI (gm/cm2) 0.54 ±0.01 0.44 ±0.01a 0.51 ±0.01bc Data was expressed as Mean ±SEM. a P0.05 in comparison to the intact control group. c P Table-2: Changes in metabolic parameters among different groups Intact control Hyperthyroid control Hyperthyroid PTU treated Glucose (mg/dl) 91.5 ±0.92 113 ±2.33a 91.33 ±1.2bc Insulin (à ¯Ã‚ Ã‚ ­IU/ml) 2.95 ±0.12 4.86 ±0.16a 2.88 ±0.13bc HOMA-IR 0.67 ±0.02 1.36 ±0.05a 0.65 ±0.03bc TC (mg/dl) 106.5 ±2.51 89.83 ±2.52a 103.33 ±1.63bd TG (mg/dl) 55.17 ±1.22 52.33 ±0.67b 53.17 ±0.95be HDL-C (mg/dl) 54 ±1.15 43.33 ±0.88a 51.33 ±0.99bc LDL-C (mg/dl) 41.47 ±3.27 36.03 ±3.07b 41.37 ±1.92be Data was expressed as Mean ±SEM. a P0.05 in comparison to the intact control group. c P0.05 in comparison to the hyperthyroid control group. HOMA-IR, homeostasis model assessment of insulin resistance index; TC, total cholesterol; TG, triglycerides; HDL-C, high density lipoprotein-cholesterol; LDL-C, low density lipoprotein-cholesterol. Figure-2: Correlations between serum irisin levels and: TSH, T4 T3 in, the intact control group (A), hyperthyroid control group (B) and hyperthyroid PTU treated group (C); glucose in the intact control group (D); insulin HOMA-IR in the intact control group (E); glucose in the hyperthyroid control group (F); insulin HOMA-IR in the hyperthyroid PTU treated group (G); TC HDL-C in the hyperthyroid control group (H). r is the correlation coefficient. Table-1 showed changes in serum levels of TSH, T4, T3 irisin, and, BMI among different groups. In the hyperthyroid control group, there was a significant decrease in serum level of TSH (0.81 ±0.05, P group (2.82 ±0.12), (0.54 ±0.01) (5.33 ±0.14), (1.29 ±0.04) and (401.67 ±5.65) respectively. On the other hand, there were insignificant changes (P>0.05) in serum levels of TSH, T4, T3 irisin, and, BMI in hyperthyroid PTU group in comparison to that in the intact control group. Also, there was a significant increase in both serum level of TSH (2.68 ±0.14, P0.05) in the hyperthyroid PTU treated group in comparison to that in the intact control group. Moreover, there was a significant decrease in the serum levels of both glucose (91.33 ±1.2, P0.05) in serum levels of both TG (52.33 ±0.67) and LDL-C (36.03 ±3.07) in comparison to that in the intact control group (106.5 ±2.51), (54 ±1.15), (55.17 ±1.22) and (41.47 ±3.27) respectively. In the hyperthyroid PTU treated group, there was a significant increase in serum levels of both TC (103.33 ±1.63, P0.05) in serum levels of both TG (53.17 ±0.95) and LDL-C (41.37 ±1.92) in comparison to that in the intact co ntrol group (89.83 ±2.52), (43.33 ±0.88), (52.33 ±0.67) and (36.03 ±3.07) respectively. Also, there were no significant changes (P>0.05) in serum levels of TC, TG, HDL-C and LDL-C in the hyperthyroid PTU treated group in comparison to that in the intact control group. Figure-2A showed a significant positive correlation between serum irisin levels and that of T4 (r=0.97, P Discussion: This study was done to declare changes in irisin serum levels in a rat model of hyperthyroidism and the effect of PTU therapy on it. Also, to identify the association between serum irisin level changes in such conditions and some metabolic parameters including insulin resistance and lipid profile. The results of this study showed a significant decrease in serum level of TSH and BMI, but, a significant increase in serum levels of T4, T3, and irisin in the hyperthyroid control group, in comparison to that in the intact control group. Also, there was a significant positive correlation between serum irisin levels and that of T4 T3 but a significant negative correlation with serum TSH in the hyperthyroid control group. These results were supported by Huh, Panagiotou et al. (2012) who stated that FNDC5, irisin precursor, was present in the thyroid tissue. Also, the present study results were supported by Irrcher, Adhihetty et al. (2003), Ruchala, Zybek et al. (2014), Sanchis-Gomar and Per ez-Quilis (2014), Samy, Ismail et al. (2015) and Yalcin, Akturk et al. (2016) who found an increase in serum irisin levels in hyperthyroidism and they suggested that it might contribute to altered energy metabolism. Moreover, Irrcher, Adhihetty et al. (2003) reported that T3 increased the irisin precursor, PGC-1ÃŽÂ ±, expression in muscle. Furthermore, Sanchis-Gomar and Perez-Quilis (2014) stated that serum irisin was increased in hyperthyroidism as a compensatory response to oxidative stress as it had an antioxidant effect. On the contrary, Gouni-Berthold, Berthold et al. (2013), Stengel, Hofmann et al. (2013) and Panagiotou, Pazaitou-Panayiotou et al. (2016) stated that changes in levels of thyroid hormones did not affect circulating irisin levels in humans. This discrepancy between their results and that of this study could be explained by species difference. Regarding the negative correlation between both irisin and TSH hormones which was present in this study, it was support ed by Ruchala, Zybek et al. (2014). On the contrary, Stengel, Hofmann et al. (2013) and Ellefsen, Vikmoen et al. (2014) reported lack of correlation between serum levels of irisin and that of TSH and thyroid hormones. Regarding body weight changes with hyperthyroidism, the result of this study was supported by Janson, Karlsson et al. (1995), Voldstedlund, Tranum-Jensen et al. (1995) and Loeb (1996) who found a decrease in body weight with hyperthyroidism. Also, this study results were supported by Bostrà ¶m, Wu et al. (2012), Castillo-Quan (2012) and Roca-Rivada, Castelao et al. (2013) who found that irisin had an anti-obesity effect as it replaced the white adipose tissue with brown adipose tissue and increased oxygen consumption and thermogenesis. On the contrary, Stengel, Hofmann et al. (2013) and Saleh, et al. (2014) found that irisin level was high in obesity and low in anorexia nervosa. This discrepancy between their results and that of this study can be explained by species difference. On the other hand, this study results showed insignificant changes in serum levels of TSH, T4, T3 irisin, and, BMI in hyperthyroid PTU group in comparison to that in the intact control group. This indicated the improvement that occurred with PTU therapy in cases of hyperthyroidism which was supported by Nakamura, Noh et al. (2007), Panda and Kar (2007) and Bahn, Burch et al. (2009). Also, the results of this study showed a significant increase in both serum level of TSH and BMI, but, a significant decrease in serum levels of T4, T3, and irisin in the hyperthyroid PTU treated group in comparison to that in the hyperthyroid control group. These results were supported by Park, Lee et al. (2016) who found that PTU administration ameliorated hyperthyroidism, reducing T4 and T3, and, increasing both TSH and BMI. Moreover, the results of this study showed a significant increase in the serum level of both glucose and insulin, and, the value of HOMA-IR in the hyperthyroid contro l group in comparison to that of the intact control group. Also, there was a significant positive correlation between serum irisin levels and that of glucose in the hyperthyroid control group. These results indicated occurrence of insulin resistance with hyperthyroidism which was supported by Loeb (1996). This was supported also by Saleh, et al. (2014) who stated that hyperglycemia gradually up regulated FNDC5/irisin expression in the skeletal muscles non-diabetic persons. Also, this study results were supported by Hee Park, Zaichenko et al. (2013), Liu, Wong et al. (2013) and Mehrabian, Taheri et al. (2016) who found a positive correlation between serum irisin and that of glucose. On the contrary, Mehrabian, Taheri et al. (2016) found that serum irisin was negatively correlated with serum glucose level. Opposite to the results of this study, Huh, Panagiotou et al. (2012), Choi, Kim et al. (2013) and Sanchis-Gomar and Perez-Quilis (2014) found that decreased blood irisin level was a ccompanied by insulin resistance development. Sanchis-Gomar and Perez-Quilis (2014) stated also, that irisin ameliorated insulin resistance by increasing betatrophin hormone expression which stimulated ÃŽÂ ²-cell regeneration. Furthermore, the results of this study showed no significant changes in the serum level of both glucose and insulin, and, the value of HOMA-IR in the hyperthyroid PTU treated group in comparison to that in the intact control group. Also, there was a significant decrease in the serum levels of both glucose and insulin, and, the value of HOMA-IR in the hyperthyroid PTU treated group in comparison to that of the hyperthyroid control group which means improvement of insulin resistance with PTU treatment. On the other hand, Huh, Panagiotou et al. (2012) found that exposure to high blood glucose for a long time was associated with a significant decrease in irisin serum level. The discrepancy between their results and the result of this study could be explained by the difference in species and duration of the study. In the hyperthyroid control group, there was a significant decrease in serum levels of both TC and HDL-C but, there were insignificant changes in serum levels of both TG and LDL-C in comparison to that in the intact control group. Also, there were significant negative correlations between serum irisin levels and that of both TC and HDL-C in the hyperthyroid control group. On the other hand, in the hyperthyroid PTU treated group, there was a significant increase in serum levels of both TC and HDL-C but, there were insignificant changes in serum levels of both TG and LDL-C in comparison to that in the intact control group. Also, there were no significant changes in serum levels of TC, TG, HDL-C and LDL-C in the hyperthyroid PTU treated group in comparison to that in the intact control group. The results of this study was partly supported by Mehrabian, Taheri et al. (2016) found a negative correlation between irisin and TC but, a po sitive correlation with HDL-C in normal weight obesity. On the other hand, Liu, Wong et al. (2013) found a positive correlation between serum irisin level and TC in non- obese, non- diabetic persons. Also, Sanchis-Gomar, Alis et al. (2014) reported insignificant correlation between irisin and TC. Conclusion   Ã‚   This study showed a significant increase in serum levels of irisin in L-thyroxine induced hyperthyroidism in rats and this could be related to changes in thyroid hormones, body weight and metabolic parameters. On using PTU, serum levels of irisin, thyroid hormones, metabolic parameters and BMI were significantly changed in comparison to the hyperthyroid control group and insignificantly changed in comparison to the intact control group which confirmed the improvement of the hyperthyroidism. Future studies are needed to confirm these results and to ensure the possible use of irisin as a biomarker for proper treatment of hyperthyroidism.

Fashion and Semiotics Essay -- Sociology Semiology Symbolism

Ever since their invention many centuries ago, clothes have been used as a way of communicating. The message communicated relies on a number of factors including the social background of both the communicator and the receiver, and the context in which the message is communicated. Although at times the exact message or symbolism one is trying to portray may not be clear, it is evident that clothing has long been embraced as one of the best ways to project one’s desired personal image to those around them. For many centuries clothing was used namely as a form of symbolising one’s ascribed class and social honour. A good example of this was evident in Feudal European times when sumptuary laws were created in order to regulate and specify the clothing that could be worn by certain classes. In 1463 Edward IV went so far as to ‘[declare] that purple silk was to be the prerogative of the aristocracy’ (Finkelstein 1991, pg. 137). As purple dye and silk were both very expensive and sought after this declaration demonstrated quite simply that those who were in possession of such materials should command respect and were of high social standing. Eventually these laws were abolished as, instead of ‘confining people to their designated rank, the laws provoked an intense interest in fashion and a desire to transgress the codes, both in the process of prestigious emulation and as an act of rebellion’ (Craik, 1994, pg. 205). This abolition allowed groups and individu als to establish their own chosen style or ‘marker’ in order to indicate their place within society. By allowing such freedom, ascribed social status gave way to that which was achieved. This not only meant that many more people were able to engage in the ever-expanding culture of ‘Haute couture’ but also that honour was no longer perceived as a birth right but rather as something that could to be obtained. Such a shift in symbolism provided a way for those of not so noble a birth to portray themselves as the latter through a variety of means such as renting or stealing clothes and buying counterfeit copies (a common occurrence in today’s society also). In the late 18th century the Industrial Revolution occurred causing a huge shift in the ways in which clothing was produced and subsequently altering the ways in which clothing was perceived. For decades preceding industrialisation men and women of high so... ...o contextual influences and past experiences. Therefore it is almost impossible to pre-empt the ways in which others will perceive you. Over time ideas will change and therefore alter the ways in which we look back on past clothing choices. Whilst symbolism in fashion may no longer be subject to laws as it was in the 14th century or defined by strict social rules in the 17th century, the clothes we wear are still today subject to imposed social ideals. Apart from distinguishing one status group from another, a style of dress may also aid group cohesiveness, provide the individual with an identity and a feeling of belonging, and communicate the wearer’s attitudes and interests. The ways in which we interpret others and present ourselves for interpretation is the only true way that we can be individual. Symbolism in clothing may not seem as obvious or important nowadays as it was in times of extreme social bigotry, but it is still highly prevalent and has remained one of the most effective ways to project our desired image to those around us. ‘The state of a person’s clothes is synonymous with self respect and is a sign of responsibility’ (www. Pemberley.com/janeinfo/vebleis7.html)

Communications and Information Technology and Legislation

Aistear is the curriculum framework for children from birth to six years. The Framework helps children grow and develop as confident and competent learners through fun, interesting and challenging experiences. The Aistear Toolkit is the place where you can access lots of practical resources online to help you get started with using Aistear. One of the topics discussed on the Aistear toolkit is learning through play. Children love to play and these resources will help a teacher to support children to learn through their play as suggested throughout the curriculum. We will now review one of the Aistear videos named Planning and Organising Play. The video is produced by an infant teacher. The teacher looks at pedagogical framing and her experiences of planning and organising her own classroom. There are many excellent suggestions as to how to set up play in a class room environment which I will explain next, however there are one or two weaknesses that if I was given the opportunity I would change to this video. Overall the video is very helpful and it does give excellent advice from an experienced infant teacher that would be like gold dust to an inexperienced teacher new to this profession. We will name the teacher Mary for the purpose of this critique as Aistear does not state the name of the presenter. Mary looks at pedagogical framing which she states lays the foundations for play. She calls it behind the scene work. According to Mary it includes the provision of resources, arrangement of space, and the establishment of daily routines, thematic planning and assessment. Mary communicates her message across in an orderly and mannerly tone. She is easy to listen to and reminds me of a teacher that I had many years ago wise and well experienced. I would be confident to implement her suggestions just by the tone of her voice. If I had one negative point in relation to the speaker that would be that she does not appear at any point throughout the video. It would be nice to be able to connect with her as she explains her view. The video has many strengths and I found many parts very helpful for planning play activities in the past. Mary uses her past experiences to help give advice with planning and organising a class room. The video starts by recommending different play areas that could be introduced into the classroom, Mary recommends that the teacher should choose five or six areas for the academic year. Such as sand area, water area, block area, small world, creative and social dramatic areas. Mary takes each play area that she recommends and explains the benefits of using these for the children’s learning and development. Using her experience she explains that the sand area works very well in her classroom, she goes on to explain that sand areas are excellent for small group play and that it encourages the children to use their imagination. Her advice is very helpful when she suggests that the children should select other resources from other types of play e. g. small world. I found her suggestions very helpful when planning my own activities and my learning goals were achieved when using the ideas from this video. The video explains the learning goals that can be achieved using these play areas such as having a sand area in the class room creates a learning environment, children learn sorting, counting and learn mathematical questions naturally. When I was planning activities and trying to introduce the Aistear themes such as Exploring and Thinking I introduced the sand activity to help the children understand concepts such as matching, ordering, counting, sorting, and capacity in an enjoyable and meaningful way. Mary explains that by using a water area for play it arouses the children’s curiosity and that whilst children play in the water area they are verbally interacting together. Asking questions and making comments bring forward ideas and suggestions and helps groups agree on solutions. She talks about real life or imaginative experiences using the blocks area. That play must be purposeful. This is very relevant to Aistear as throughout the Aistear curriculum it is suggested that play must have meaning so that the children will achieve there learning goals. Mary explains all the areas of play in great detail and links are evident throughout that the Aistear Curriculum is at the heart of this video, however if you as a viewer were not familiar with the curriculum and were using this video to help you to create your activity plans your plans would not meet the level that the curriculum would expect because the video does not make any distinct links to the actual layout of the Aistear curriculum. She does not mention the themes such as Well-being, Identity and Belonging, Communicating or Exploring and Thinking. The play areas that are mentioned do cover the learning goals for the themes and for an inexperienced person training to be a teacher they would not make this connection. If the video had captions throughout of the themes it would link the curriculum and the video perfectly. The Curriculum is designed around different age groups (birth to six years). The video does not mention the age groups or how each play area could be used for the different age categories. Throughout the Aistear Curriculum examples are given for babies, toddlers and young children and Mary does not implement this important distinction in the video once. The video suggests types of play that are low cost for materials and easy to set up using everyday items. The teacher recommends that the play areas be changed annually to prevent boredom for the teacher and the children. However the teacher recommends that the socio dramatic area should always be present in the classroom but does not explain why. Mary talks about the physical layout of the classroom finally which I feel was not necessary as there is a separate video in the toolkit which explores this topic in great detail. The video in my opinion is not long enough for this topic to be mentioned. This time could have been allocated to the Aistear themes and how they were achieved using the play areas discussed throughout the video. Overall the video has many useful ideas that can easily be taken and used to create a playful environment but I feel from all the information available to me that there is not a strong enough connection between the actually hard copy of Aistear and the video. References: Aistear Curriculum www.ncca.ie Aistear Toolkit www.ncca.ie/aisteartoolkit‎ Video: Planning and Organising Play http://vimeo.com/21112275

The Throw-Away Society

The throw-away society From lumpish walkman to handy ipod, from family traditional wedding dress to one-time use paper gown, our society witnesses the globally social revolution of throwing away. Quantity replaces quality and hence becomes the buzzword of the new world. But looking forward and in retrospect, do we really should flood in the throw-away fashion upon which our attitude towards man-made things has altered so much? And what on earth do we throw away? When we throw away the no-more-need things and replenish our refrigerators and wardrobes, we throw away resources. No matter they are patches of fabric to make a new scarf or tons of steel to produce new cars, all of them come from the valuable resources of which the stock depletes severely day by day. News and reports broadcast how scarce our natural resources are becoming, but people still throw away. Few of them have realized that the process of throwing away is the exact procedure to turn resources towards garbages. Furthermore, it is also sometimes too obscure to be noticed that throwing away products also incurs throwing away good memories. A doll you had when you were a little girl would always prompt you the innocent and sweet childhood; an umbrella you used for 4 years in the campus would always remind you of the fresh and brisk university life. When all these similar reminders become less and less, that particular period of life would be more likely to be locked somewhere in your mind, not so easy to be touched again any more. Admittedly, the throw-away society gives us so many choices among which we are prone to be less picky but also more emotionless. We seldom keep a dress for 10 years like our mothers do since all the new styles have emerged and we have so many chances to choose a more delicate one. When we discard the old one, we don’t feel a tiny bit mercy; when we welcome the new one, we don’t sense the joy that much either. It’s believed today that the whole world, material or non-material, is formulated this way, easy and convenient; thus we tend to be less reminiscent of the unforgettable birthday party while we were attired with that particular dress. The dress is still the original one, but the connotation of the dress has changed through your life, for it has been a witness to your growth, your maturity, tear and laughter, ups and downs. The longer you keep it, the more it becomes part of yourself. So many people nowadays complain that they are lost somewhere in their life and they feel onfused and puzzled to forge ahead. It’s a paradox that people want to sustain a whole self but still discard part of himself. Living in the throw-away society, what people eventually throw away is the real self, subconsciously and accidentally. It’s a trend that we will continue throwing away, as it seems to be destined. But during this inexorable process, more reflections are bound to elaborat e and expand on it since we all despise to see a world with complete trash but desperate ourselves.

Alone Together Professor Ramos Blog

Alone Together Have you ever felt alone, even for just a split second? If you ever read any of Sandra Cisneros’ work, you can constantly see the theme of loneliness throughout many of her short stories. Perhaps this theme stems from her life of always being alone. During an interview with Pilar E. Aranda Rodriguez for The Americas Review, Mr. Rodriguez asked Cisneros why she has never married. Cisneros replies, â€Å"Ive never seen a marriage that is as happy as my living alone. My writing is my child and I dont want anything to come between us† (Rodriguez). She seems very comfortable with being alone, yet in the stories she portrays how loneliness is actually supposed to feel like. In the stories â€Å"There is a Man, There is a Woman,† â€Å"Woman Hollering Creek,† and â€Å"Never Marry a Mexican,† one can see how they are all connected through the theme of loneliness, but each character hides their feelings with their actions. The way loneliness is portrayed in the short story â€Å"There is a man, There is a woman† can be expressed through the characters’ everyday life. The man and the woman’s friends cannot see how they truly feel and how hurt they are. For example, when the man and the woman drink with their friends on Friday’s, they drink hoping they would say something but never say it (Cisneros). Maybe they are trying to express how lonely they feel, yet they can never open up to close friends. Its hard to believe how people could be doing the same thing right at the same moment, in the same place but they will most likely never meet. Although the man and woman share the same actions whenever there doing something, it seems very cruel that they will never know about each other. The theme of this story revolves around the idea that being alone doesnt always mean that you are really alone because someone out there can be feeling the same thing you are feeling. In other words , your connected through the sense of loneliness.   In the story titled â€Å"Woman Hollering Creek,† loneliness is hidden through the violence of Cleofà ­las marriage. Being alone seems to be growing upon the main character, Cleofà ­las, as the story goes on. In the beginning of â€Å"Woman Hollering Creek,† it is as if Cleofà ­las is living that great way of life as all other great marriages. She was getting married, having a child, moving to another country, and everything seems to be going well. That is until her husband started to beat her. When he first hits her, she thought that the next time he did, she would do something about it. Yet, she never had it in her to defend herself. Then every time she gets hit, she progressively feels lonely as if her happiness was just what you feel in the start of the marriage. Than her true emotions begin to overwhelm her. Even after having one child and being pregnant with another, Cleofà ­las can feel less and less close to the man she married as if he just a fake lover. Al one with her kids she would push herself to do the unthinkable and would finally leave her husband. In spite of Cleofà ­las’ feeling of happiness and gratitude toward her children, she cannot hide how truly lonely she has become through her marriage. It’s clear how this story also shares the theme of loneliness, but is hidden with the characters actions.   Finally, the last story that takes part in the theme of loneliness is â€Å"Never Marry a Mexican.† This is a complicated story that is nevertheless a sad or appalling one, depending on your point of view. The writing is basically about a woman named Clemencia who enjoyed sleeping with married men. Although you could argue that she picked this up from her mother since the mother cheated on her dying husband when Clemencia was young, yet Clemencia is still an adult and has the option to say â€Å"no† to every situation. You can also argue that she was targeted by married men, but could have rejected them. Perhaps she feels like she needs to make bad decisions in order to feel less alone. Her first affair occurred while she was in college with a Professor who was married. Clemencia would grow to love this Professor but she knew that she would never truly be happy with him because as a young child, she saw from her mom that there was no such thing as true love. So, after d enying the Professor of his offer to leave his wife for her, she would later regret her decision and try to fix her mistake but it was already too late because the Professor would never see her the same way again. Clemencia would then want revenge so she waited years to sleep with the Professors son. Throughout the whole story, Clemencia constantly enjoys her way of her life yet, she’s also trying to find love but was not able to. Even at the end of the story she said â€Å"I just want to reach out and stroke someone, and say There, there it’s all right, honey. There, there, there† (Cisneros). Clearly, she wanted to be with some but was truly alone. As if her purpose was just to create holes in marriages because she had no way of actually dealing with being lonely. As you can see, loneliness is a significant theme in this story but Clemencia covers this feeling up with her terrible deeds.   Being alone can be a very brutal thing. It is as if you were born just to be born and to never know a person that is close to you, without you even knowing. In other words, you feel like you have no purpose but there is someone out there that feels exactly the same way, you just might never meet because of the path you are in. You could say that its fate or just bad luck. In the end you still feel that empty spot in which someone is going to fulfill one day, but has not fulfilled yet. The thought of never understanding why you still feel lonely builds more and more whenever you think of your state of being alone. Almost as if the wound that was created by your past actions makes you more careless yet, scares you at the same time. That unwanted thought of being alone fills your mind and changes the way you look at life. In the stories â€Å"There is a Man, There is a Woman,† â€Å"Woman Hollering Creek,† and â€Å"Never Marry a Mexican,† each main character has a way of covering up their loneliness through their actions such as drinking with friends, pretending to think ones marriage will change, or sleeping with married men. Which concludes that loneliness plays a great deal on how people act in certain situations. Perhaps the only way of stopping this feeling of loneliness is through companionship, which each character is seeking. Bibliography Cisneros, Sandra. Woman Hollering Creek and Other Stories. First Vintage Contemporaries Edition, March 1992, Kindle Cloud Reader, read.amazon.com. Rodrà ­guez Aranda, Pilar E. â€Å"On the Solitary Fate of Being Mexican, Female, Wicked and Thirty-Three: An Interview with Writer Sandra Cisneros.† The Americas Review, 1990, pp. 65–80.

The case study 1-1 lincon Electric Essay Example | Topics and Well Written Essays - 3000 words

The case study 1-1 lincon Electric - Essay Example 33) Use module theory and evidence from the firm’s past experience to explore the advantages and disadvantages of these entry modes. Which would you recommend for use in Lincoln’s entry to India, and why†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.†¦Ã¢â‚¬ ¦.......................................................................................................................7 How far and in what ways have Lincoln Electric developed dynamic capabilities through learning form experience in Japan, South Korea and China? Apply your analysis to explain what changes the firm will need to make to its resource management strategy in order to succeed in India†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦...†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.....12 Appendix 1- Bibliographyâ € ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦...16 Section A Identify and appraise the key strategic issues confronting John Stropki as he leads the expansion of Lincoln Electric’s international Business in 2006. ... John Stropki, the Chairman, Chief Executive Officer and the President of the company, has played a key role in expanding the company’s business globally, and now he is planning to expand the operation in India in 2006, after covering the markets in North America, Latin America, Europe and Asia Pacific. The company has expanded its operation worldwide through joint ventures, acquisitions and establishing its own plants through out the globe as a major part of its strategy. In its present strategy the company has set a range of financial goals which are not likely to be satisfied only by its operations in the home market of United States. The company’s revenue are still derived mostly form North America; where as the market of welding industry is growing fast all over the globe. Company’s latest goals include double sales growth as compared to the worldwide industrial production, operational margins of over 15% earnings growth of 10% annually, and return on equity of more than 20%. In order to achieve these goals the company had spent approximately two thirds of its free cash flow on global expansion. Now the CEO/Chairman and also the President of the company is focusing on its experience in China and how he can use it so that the same mistakes are not repeated. According to Kendizor, there are a certain kinds of factors that a company should consider when it’s set on the course of expanding its operations globally. Business Risk: The Business risk is high as the stability of the business overall structure depends on how the results would turn up. We have seen in the case of Lincoln Electric in the year 1999, that whenever it entered a global market unprepared it has suffered huge losses, not only internationally but locally as well. Financial Risk: Though

The Social Contract Theory and Animals Essay Example | Topics and Well Written Essays - 1000 words

The Social Contract Theory and Animals - Essay Example This essay stresses that human beings have no known form of direct duty to the animals. Only an individual who has a moral duty cannot be able to have a moral obligation. From the above understanding, animals have no moral duty and hence, no moral obligation. Both human beings and animals would have interest but the interests of human beings in the social contract theory override those of the animals. As such, if the human beings have an interest to kill and eat animals, that interest would be morally justified and it would override the interests that animals have to stay alive. No morals would have been broken in this case. This paper makes a conclusion that only rational beings can be in a position to enter into a social contract. Those beings that are not rational, that is the animals, lack this standing. The rational beings have moral duties and responsibilities that they are required to adhere to. On the contract, animals lack these moral duties and responsibilities. It would, therefore, be proper to argued that animals have no moral standing whatsoever and they cannot take part in a social contract. The form of protection given to human beings under the social contract by the government cannot possibly be given to the animals as well. The animals are still in a state of nature and they have no moral values guiding their day to day activities.